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Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
BACKGROUND: The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic ‘safe harbor’ site, have not been prod...
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| Pubblicato in: | BMC Biotechnol |
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| Autori principali: | , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4715285/ https://ncbi.nlm.nih.gov/pubmed/26772810 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-016-0234-4 |
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