Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

BACKGROUND: The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic ‘safe harbor’ site, have not been prod...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Chu, Van Trung, Weber, Timm, Graf, Robin, Sommermann, Thomas, Petsch, Kerstin, Sack, Ulrike, Volchkov, Pavel, Rajewsky, Klaus, Kühn, Ralf
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4715285/
https://ncbi.nlm.nih.gov/pubmed/26772810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-016-0234-4
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