Live-cell super-resolved PAINT imaging of pN cellular traction forces

Despite the vital role of mechanical forces in biology, no technique has been reported to image cellular force with sub-100 nm resolution. Here, we present tension-PAINT (tPAINT), integrating molecular tension probes with the DNA-PAINT technique to map pN mechanical events with ~25 nm resolution. To...

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Détails bibliographiques
Publié dans:Nat Methods
Auteurs principaux: Brockman, Joshua M., Su, Hanquan, Blanchard, Aaron T., Duan, Yuxin, Meyer, Travis, Quach, M. Edward, Glazier, Roxanne, Bazrafshan, Alisina, Bender, Rachel L., Kellner, Anna V., Ogasawara, Hiroaki, Ma, Rong, Schueder, Florian, Petrich, Brian G., Jungmann, Ralf, Li, Renhao, Mattheyses, Alexa L., Ke, Yonggang, Salaita, Khalid
Format: Artigo
Langue:Inglês
Publié: 2020
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7574592/
https://ncbi.nlm.nih.gov/pubmed/32929270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-020-0929-2
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