Live-cell super-resolved PAINT imaging of pN cellular traction forces

Despite the vital role of mechanical forces in biology, no technique has been reported to image cellular force with sub-100 nm resolution. Here, we present tension-PAINT (tPAINT), integrating molecular tension probes with the DNA-PAINT technique to map pN mechanical events with ~25 nm resolution. To...

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Publicado no:Nat Methods
Main Authors: Brockman, Joshua M., Su, Hanquan, Blanchard, Aaron T., Duan, Yuxin, Meyer, Travis, Quach, M. Edward, Glazier, Roxanne, Bazrafshan, Alisina, Bender, Rachel L., Kellner, Anna V., Ogasawara, Hiroaki, Ma, Rong, Schueder, Florian, Petrich, Brian G., Jungmann, Ralf, Li, Renhao, Mattheyses, Alexa L., Ke, Yonggang, Salaita, Khalid
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7574592/
https://ncbi.nlm.nih.gov/pubmed/32929270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-020-0929-2
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