Live-cell super-resolved PAINT imaging of pN cellular traction forces

Despite the vital role of mechanical forces in biology, no technique has been reported to image cellular force with sub-100 nm resolution. Here, we present tension-PAINT (tPAINT), integrating molecular tension probes with the DNA-PAINT technique to map pN mechanical events with ~25 nm resolution. To...

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Pubblicato in:Nat Methods
Autori principali: Brockman, Joshua M., Su, Hanquan, Blanchard, Aaron T., Duan, Yuxin, Meyer, Travis, Quach, M. Edward, Glazier, Roxanne, Bazrafshan, Alisina, Bender, Rachel L., Kellner, Anna V., Ogasawara, Hiroaki, Ma, Rong, Schueder, Florian, Petrich, Brian G., Jungmann, Ralf, Li, Renhao, Mattheyses, Alexa L., Ke, Yonggang, Salaita, Khalid
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2020
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7574592/
https://ncbi.nlm.nih.gov/pubmed/32929270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-020-0929-2
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