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Generalized enzymatic mechanism of catalysis by tetrameric l-asparaginases from mesophilic bacteria

The mechanism of catalysis by the l-glutaminase-asparaginase from Pseudomonas 7A (PGA) was investigated using structural, mass spectrometry, and kinetic data. We had previously proposed mechanism of hydrolysis of l-Asn by the type II l-asparaginase from E. coli (EcAII), but that work was limited to...

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Podrobná bibliografie
Vydáno v:Sci Rep
Hlavní autoři: Strzelczyk, Pawel, Zhang, Di, Dyba, Marzena, Wlodawer, Alexander, Lubkowski, Jacek
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7567106/
https://ncbi.nlm.nih.gov/pubmed/33060684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-74480-4
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