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Dynamic High-Sensitivity Quantitation of Procollagen-I by Endogenous CRISPR-Cas9 NanoLuciferase Tagging

The ability to quantitate a protein of interest temporally and spatially at subcellular resolution in living cells would generate new opportunities for research and drug discovery, but remains a major technical challenge. Here, we describe dynamic, high-sensitivity protein quantitation technique usi...

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Vydáno v:Cells
Hlavní autoři: Calverley, Ben C., Kadler, Karl E., Pickard, Adam
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7564849/
https://ncbi.nlm.nih.gov/pubmed/32927811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9092070
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