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A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3′end using CRISPR-Cas9 genome editing technique

α-synuclein (α-SYN) is a major pathologic contributor to Parkinson’s disease (PD). Multiplication of α-SYN encoding gene (SNCA) is correlated with early onset of the disease underlining the significance of its transcriptional regulation. Thus, monitoring endogenous transcription of SNCA is of utmost...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Basu, Sambuddha, Adams, Levi, Guhathakurta, Subhrangshu, Kim, Yoon-Seong
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2017
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5379209/
https://ncbi.nlm.nih.gov/pubmed/28374838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep45883
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