ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons

The correct subcellular distribution of proteins establishes the complex morphology and function of neurons. Fluorescence microscopy techniques are invaluable to investigate subcellular protein distribution, but they suffer from the limited ability to efficiently and reliably label endogenous protei...

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Detalhes bibliográficos
Publicado no:PLoS Biol
Main Authors: Willems, Jelmer, de Jong, Arthur P. H., Scheefhals, Nicky, Mertens, Eline, Catsburg, Lisa A. E., Poorthuis, Rogier B., de Winter, Fred, Verhaagen, Joost, Meye, Frank J., MacGillavry, Harold D.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2020
Assuntos:
Methods and Resources
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7176289/
https://ncbi.nlm.nih.gov/pubmed/32275651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pbio.3000665
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