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Multiplex enCas12a screens detect functional buffering among paralogs otherwise masked in monogenic Cas9 knockout screens

BACKGROUND: Pooled library CRISPR/Cas9 knockout screening across hundreds of cell lines has identified genes whose disruption leads to fitness defects, a critical step in identifying candidate cancer targets. However, the number of essential genes detected from these monogenic knockout screens is lo...

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Publicat a:Genome Biol
Autors principals: Dede, Merve, McLaughlin, Megan, Kim, Eiru, Hart, Traver
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7558751/
https://ncbi.nlm.nih.gov/pubmed/33059726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02173-2
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