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Unbiased investigation of specificities of prime editing systems in human cells

Prime editors (PEs) enable targeted precise editing, including the generation of substitutions, insertions and deletions, in eukaryotic genomes. However, their genome-wide specificity has not been explored. Here, we developed Nickase-based Digenome-seq (nDigenome-seq), an in vitro assay that uses wh...

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Библиографические подробности
Опубликовано в: :Nucleic Acids Res
Главные авторы: Kim, Do Yon, Moon, Su Bin, Ko, Jeong-Heon, Kim, Yong-Sam, Kim, Daesik
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2020
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7544197/
https://ncbi.nlm.nih.gov/pubmed/32941652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa764
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