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Unbiased investigation of specificities of prime editing systems in human cells

Prime editors (PEs) enable targeted precise editing, including the generation of substitutions, insertions and deletions, in eukaryotic genomes. However, their genome-wide specificity has not been explored. Here, we developed Nickase-based Digenome-seq (nDigenome-seq), an in vitro assay that uses wh...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Kim, Do Yon, Moon, Su Bin, Ko, Jeong-Heon, Kim, Yong-Sam, Kim, Daesik
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2020
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7544197/
https://ncbi.nlm.nih.gov/pubmed/32941652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa764
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