Promoter Orientation within an AAV-CRISPR Vector Affects Cas9 Expression and Gene Editing Efficiency

Adeno-associated virus (AAV) vectors have been widely adopted for delivery of CRISPR-Cas components, especially for therapeutic gene editing. For a single vector system, both the Cas9 and guide RNA (gRNA) are encoded within a single transgene, usually from separate promoters. Careful design of this...

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Detalles Bibliográficos
Publicado en:CRISPR J
Main Authors: Fry, Lewis E., Peddle, Caroline F., Stevanovic, Marta, Barnard, Alun R., McClements, Michelle E., MacLaren, Robert E.
Formato: Artigo
Idioma:Inglês
Publicado: Mary Ann Liebert, Inc., publishers 2020
Assuntos:
Research Articles
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7469699/
https://ncbi.nlm.nih.gov/pubmed/32833533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2020.0021
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