Promoter Orientation within an AAV-CRISPR Vector Affects Cas9 Expression and Gene Editing Efficiency

Adeno-associated virus (AAV) vectors have been widely adopted for delivery of CRISPR-Cas components, especially for therapeutic gene editing. For a single vector system, both the Cas9 and guide RNA (gRNA) are encoded within a single transgene, usually from separate promoters. Careful design of this...

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Spremljeno u:
Bibliografski detalji
Izdano u:CRISPR J
Glavni autori: Fry, Lewis E., Peddle, Caroline F., Stevanovic, Marta, Barnard, Alun R., McClements, Michelle E., MacLaren, Robert E.
Format: Artigo
Jezik:Inglês
Izdano: Mary Ann Liebert, Inc., publishers 2020
Teme:
Research Articles
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7469699/
https://ncbi.nlm.nih.gov/pubmed/32833533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2020.0021
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