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Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence
CRISPR/Cas-mediated genome editing is a powerful tool for generating genetically mutated cells and organisms. Linearisation of donor cassettes with this system has been shown to facilitate both transgene donor insertion and targeted knock-in. Here, we developed a donor plasmid that we name pCriMGET...
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| Publicat a: | Sci Rep |
|---|---|
| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group UK
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7445171/ https://ncbi.nlm.nih.gov/pubmed/32839482 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-70804-6 |
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