Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

Engineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5′ end can enhance the gene editing efficiency of Cpf1 both in cells and in vivo. Extending the 5′ end of the crR...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Park, Hyo Min, Liu, Hui, Wu, Joann, Chong, Anthony, Mackley, Vanessa, Fellmann, Christof, Rao, Anirudh, Jiang, Fuguo, Chu, Hunghao, Murthy, Niren, Lee, Kunwoo
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6098076/
https://ncbi.nlm.nih.gov/pubmed/30120228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-05641-3
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