Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds

CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here, we harness...

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Dettagli Bibliografici
Pubblicato in:Genome Biol
Autori principali: Teng, Fei, Li, Jing, Cui, Tongtong, Xu, Kai, Guo, Lu, Gao, Qingqin, Feng, Guihai, Chen, Chuanyuan, Han, Dali, Zhou, Qi, Li, Wei
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2019
Soggetti:
Short Report
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6362571/
https://ncbi.nlm.nih.gov/pubmed/30717767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-019-1620-8
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