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CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity

CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detecti...

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Bibliografiske detaljer
Udgivet i:Genome Biol
Main Authors: Teng, Fei, Guo, Lu, Cui, Tongtong, Wang, Xin-Ge, Xu, Kai, Gao, Qingqin, Zhou, Qi, Li, Wei
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6604390/
https://ncbi.nlm.nih.gov/pubmed/31262344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-019-1742-z
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