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Chemical crosslinking enhances RNA immunoprecipitation for efficient identification of binding sites of proteins that photo-crosslink poorly with RNA

In eukaryotic cells, proteins that associate with RNA regulate its activity to control cellular function. To fully illuminate the basis of RNA function, it is essential to identify such RNA-associated proteins, their mode of action on RNA, and their preferred RNA targets and binding sites. By analyz...

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Bibliografski detalji
Izdano u:RNA
Glavni autori: Patton, Robert D., Sanjeev, Manu, Woodward, Lauren A., Mabin, Justin W., Bundschuh, Ralf, Singh, Guramrit
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7430673/
https://ncbi.nlm.nih.gov/pubmed/32467309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.074856.120
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