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Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis
Here we generate FRET biosensors for guanine exchange factors (GEFs) by inserting a fluorescent protein pair in a structural “hinge” common to many GEFs. Fluorescent biosensors can map the activation of signaling molecules in space and time, but it has not been possible to quantify how different act...
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| Publicado no: | Nat Chem Biol |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7388658/ https://ncbi.nlm.nih.gov/pubmed/32424303 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41589-020-0542-9 |
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