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Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity

Although high affinity binding between streptavidin and biotin is widely exploited, the accompanying low rate of dissociation prevents its use in many applications where rapid ligand release is also required. To combine extremely tight and reversible binding, we have introduced disulfide bonds into...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Marangoni, Jesse M., Wu, Sau-Ching, Fogen, Dawson, Wong, Sui-Lam, Ng, Kenneth K. S.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2020
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7385176/
https://ncbi.nlm.nih.gov/pubmed/32719366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-69357-5
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