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Directed evolution methods for overcoming trade-offs between protein activity and stability

Engineered proteins are being widely developed and employed in applications ranging from enzyme catalysts to therapeutic antibodies. Directed evolution, an iterative experimental process composed of mutagenesis and library screening, is a powerful technique for enhancing existing protein activities...

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Bibliografiske detaljer
Udgivet i:AIChE J
Main Authors: Stimple, Samuel D., Smith, Matthew D., Tessier, Peter M.
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7384606/
https://ncbi.nlm.nih.gov/pubmed/32719568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/aic.16814
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