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Directed evolution methods for overcoming trade-offs between protein activity and stability

Engineered proteins are being widely developed and employed in applications ranging from enzyme catalysts to therapeutic antibodies. Directed evolution, an iterative experimental process composed of mutagenesis and library screening, is a powerful technique for enhancing existing protein activities...

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Podrobná bibliografie
Vydáno v:AIChE J
Hlavní autoři: Stimple, Samuel D., Smith, Matthew D., Tessier, Peter M.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7384606/
https://ncbi.nlm.nih.gov/pubmed/32719568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/aic.16814
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