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Directed evolution methods for overcoming trade-offs between protein activity and stability

Engineered proteins are being widely developed and employed in applications ranging from enzyme catalysts to therapeutic antibodies. Directed evolution, an iterative experimental process composed of mutagenesis and library screening, is a powerful technique for enhancing existing protein activities...

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Publicat a:AIChE J
Autors principals: Stimple, Samuel D., Smith, Matthew D., Tessier, Peter M.
Format: Artigo
Idioma:Inglês
Publicat: 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7384606/
https://ncbi.nlm.nih.gov/pubmed/32719568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/aic.16814
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