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Directed evolution methods for overcoming trade-offs between protein activity and stability

Engineered proteins are being widely developed and employed in applications ranging from enzyme catalysts to therapeutic antibodies. Directed evolution, an iterative experimental process composed of mutagenesis and library screening, is a powerful technique for enhancing existing protein activities...

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Bibliografske podrobnosti
izdano v:AIChE J
Main Authors: Stimple, Samuel D., Smith, Matthew D., Tessier, Peter M.
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7384606/
https://ncbi.nlm.nih.gov/pubmed/32719568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/aic.16814
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