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Covalent Labeling/Mass Spectrometry of Monoclonal Antibodies with Diethylpyrocarbonate: Reaction Kinetics for Ensuring Protein Structural Integrity

Diethylpyrocarbonate (DEPC)-based covalent labeling together with mass spectrometry is a promising tool for the higher-order structural analysis of antibody therapeutics. Reliable information about antibody higher-order structure can be obtained, though, only when the protein’s structural integrity...

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Detalles Bibliográficos
Publicado en:J Am Soc Mass Spectrom
Main Authors: Limpikirati, Patanachai K., Zhao, Bo, Pan, Xiao, Eyles, Stephen J., Vachet, Richard W.
Formato: Artigo
Idioma:Inglês
Publicado: 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7370534/
https://ncbi.nlm.nih.gov/pubmed/32310649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jasms.0c00067
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