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Covalent Labeling with Diethylpyrocarbonate is Sensitive to Residue Microenvironment, Providing Improved Analysis of Protein Higher Order Structure by Mass Spectrometry

Covalent labeling with mass spectrometry is increasingly being used for the structural analysis of proteins. Diethylpyrocarbonate (DEPC) is a simple to use, commercially-available covalent labeling reagent that can readily react with a range of nucleophilic residues in proteins. We find that in inta...

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Vydáno v:Anal Chem
Hlavní autoři: Limpikirati, Patanachai, Pan, Xiao, Vachet, Richard W.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6656364/
https://ncbi.nlm.nih.gov/pubmed/31150223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b01732
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