High-fidelity SaCas9 identified by directional screening in human cells

CRISPR-Staphylococcus aureus Cas9 (CRISPR-SaCas9) has been harnessed as an effective in vivo genome-editing tool to manipulate genomes. However, off-target effects remain a major bottleneck that precludes safe and reliable applications in genome editing. Here, we characterize the off-target effects...

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Gepubliceerd in:PLoS Biol
Hoofdauteurs: Xie, Haihua, Ge, Xianglian, Yang, Fayu, Wang, Bang, Li, Shuang, Duan, Jinzhi, Lv, Xiujuan, Cheng, Congsheng, Song, Zongming, Liu, Changbao, Zhao, Junzhao, Zhang, Yu, Wu, Jinyu, Gao, Caixia, Zhang, Jinwei, Gu, Feng
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2020
Onderwerpen:
Methods and Resources
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7347106/
https://ncbi.nlm.nih.gov/pubmed/32644995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pbio.3000747
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