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Allosteric Regulation of CRISPR-Cas9 for DNA Targeting and Cleavage

The CRISPR-Cas9 system from Streptococcus pyogenes has been exploited as a programmable RNA-guided DNA targeting and editing platform. This evolutionary tool enables diverse genetic manipulations with unprecedented precision and ease. Cas9 is an allosteric enzyme, which is allosterically regulated i...

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Detalles Bibliográficos
Publicado en:Curr Opin Struct Biol
Main Authors: Zuo, Zhicheng, Liu, Jin
Formato: Artigo
Idioma:Inglês
Publicado: 2020
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7308215/
https://ncbi.nlm.nih.gov/pubmed/32070859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.sbi.2020.01.013
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