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Selection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay

The thermal shift assay is a robust method of discovering protein–ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput has been advanced greatly by the rapid progress in tandem ma...

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Bibliografske podrobnosti
izdano v:J Proteome Res
Main Authors: Li, Jiaming, Van Vranken, Jonathan G., Paulo, Joao A., Huttlin, Edward L., Gygi, Steven P.
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7294736/
https://ncbi.nlm.nih.gov/pubmed/32243163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.0c00063
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