On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria

The ability to block gene expression in bacteria with the catalytically inactive mutant of Cas9, known as dCas9, is quickly becoming a standard methodology to probe gene function, perform high-throughput screens, and engineer cells for desired purposes. Yet, we still lack a good understanding of the...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Calvo-Villamañán, Alicia, Ng, Jérome Wong, Planel, Rémi, Ménager, Hervé, Chen, Arthur, Cui, Lun, Bikard, David
Format: Artigo
Language:Inglês
Published: Oxford University Press 2020
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Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7293049/
https://ncbi.nlm.nih.gov/pubmed/32352514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa294
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