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On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria
The ability to block gene expression in bacteria with the catalytically inactive mutant of Cas9, known as dCas9, is quickly becoming a standard methodology to probe gene function, perform high-throughput screens, and engineer cells for desired purposes. Yet, we still lack a good understanding of the...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2020
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7293049/ https://ncbi.nlm.nih.gov/pubmed/32352514 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa294 |
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