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Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes

CRISPR-Cas systems employ ribonucleoprotein complexes to identify nucleic acid targets with complementarity to bound CRISPR RNAs. Analyses of the high diversification of these effector complexes suggest that they can exhibit a wide spectrum of target requirements and binding affinities. Therefore, s...

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Detalles Bibliográficos
Publicado en:Front Mol Biosci
Main Authors: Müller-Esparza, Hanna, Osorio-Valeriano, Manuel, Steube, Niklas, Thanbichler, Martin, Randau, Lennart
Formato: Artigo
Idioma:Inglês
Publicado: Frontiers Media S.A. 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7266957/
https://ncbi.nlm.nih.gov/pubmed/32528975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmolb.2020.00098
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