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CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering

Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types within pro...

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Detalhes bibliográficos
Publicado no:Mol Syst Biol
Principais autores: Anderson, Daniel Paolo, Benns, Henry James, Tate, Edward William, Child, Matthew Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7266498/
https://ncbi.nlm.nih.gov/pubmed/32485092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20209475
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