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Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs

The CRISPR-Cas9 system provides unprecedented genome editing capabilities. However, off-target effects lead to sub-optimal usage and additionally are a bottleneck in the development of therapeutic uses. Herein, we introduce the first machine learning-based approach to off-target prediction, yielding...

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Detalhes bibliográficos
Publicado no:Nat Biomed Eng
Main Authors: Listgarten, Jennifer, Weinstein, Michael, Kleinstiver, Benjamin P., Sousa, Alexander A., Joung, J. Keith, Crawford, Jake, Gao, Kevin, Hoang, Luong, Elibol, Melih, Doench, John G., Fusi, Nicolo
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6037314/
https://ncbi.nlm.nih.gov/pubmed/29998038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41551-017-0178-6
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