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CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA...

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Detalhes bibliográficos
Publicado no:Bioinformatics
Main Authors: Naito, Yuki, Hino, Kimihiro, Bono, Hidemasa, Ui-Tei, Kumiko
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4382898/
https://ncbi.nlm.nih.gov/pubmed/25414360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/bioinformatics/btu743
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