Wheat germ agglutinin–conjugated fluorescent pH sensors for visualizing proton fluxes

Small-molecule fluorescent wheat germ agglutinin (WGA) conjugates are routinely used to demarcate mammalian plasma membranes, because they bind to the cell’s glycocalyx. Here, we describe the derivatization of WGA with a pH-sensitive rhodamine fluorophore (pHRho; pKa = 7) to detect proton channel fl...

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Dades bibliogràfiques
Publicat a:J Gen Physiol
Autors principals: Zhang, Lejie, Zhang, Mei, Bellve, Karl, Fogarty, Kevin E., Castro, Maite A., Brauchi, Sebastian, Kobertz, William R.
Format: Artigo
Idioma:Inglês
Publicat: Rockefeller University Press 2020
Matèries:
Methods and Approaches
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7266149/
https://ncbi.nlm.nih.gov/pubmed/31978216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1085/jgp.201912498
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