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Wheat germ agglutinin–conjugated fluorescent pH sensors for visualizing proton fluxes
Small-molecule fluorescent wheat germ agglutinin (WGA) conjugates are routinely used to demarcate mammalian plasma membranes, because they bind to the cell’s glycocalyx. Here, we describe the derivatization of WGA with a pH-sensitive rhodamine fluorophore (pHRho; pKa = 7) to detect proton channel fl...
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| Publicado no: | J Gen Physiol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Rockefeller University Press
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7266149/ https://ncbi.nlm.nih.gov/pubmed/31978216 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1085/jgp.201912498 |
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