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Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing

Index hopping is the main cause of incorrect sample assignment of sequencing reads in multiplexed pooled libraries. We introduce a statistical model for estimating the sample index-hopping rate in multiplexed droplet-based single-cell RNA-seq data and for probabilistic inference of the true sample o...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nat Commun
Egile Nagusiak: Farouni, Rick, Djambazian, Haig, Ferri, Lorenzo E., Ragoussis, Jiannis, Najafabadi, Hamed S.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Nature Publishing Group UK 2020
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7264361/
https://ncbi.nlm.nih.gov/pubmed/32483174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-16522-z
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