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Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing
Index hopping is the main cause of incorrect sample assignment of sequencing reads in multiplexed pooled libraries. We introduce a statistical model for estimating the sample index-hopping rate in multiplexed droplet-based single-cell RNA-seq data and for probabilistic inference of the true sample o...
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| Gepubliceerd in: | Nat Commun |
|---|---|
| Hoofdauteurs: | , , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Nature Publishing Group UK
2020
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7264361/ https://ncbi.nlm.nih.gov/pubmed/32483174 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-16522-z |
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