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CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs
Genome editing has been revolutionized by the CRISPR-Cas9 system. CRISPR-Cas9 is composed of single-molecular guide RNA (sgRNA) and a proteinaceous Cas9 nuclease, which recognizes a specific target sequence and a protospacer adjacent motif (PAM) sequence and, subsequently, cleaves the targeted DNA s...
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| Publicado en: | Genome Res |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Cold Spring Harbor Laboratory Press
2020
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7263196/ https://ncbi.nlm.nih.gov/pubmed/32327447 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.257493.119 |
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