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Efficient genome editing by CRISPR-Mb3Cas12a in mice
As an alternative and complementary approach to Cas9-based genome editing, Cas12a has not been widely used in mammalian cells largely due to its strict requirement for the TTTV protospacer adjacent motif (PAM) sequence. Here, we report that Mb3Cas12a (Moraxella bovoculi AAX11_00205) can efficiently...
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| Publicado no: | J Cell Sci |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Company of Biologists Ltd
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7240296/ https://ncbi.nlm.nih.gov/pubmed/32393674 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.240705 |
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