Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes

CRISPR RNA-guided endonucleases (RGEs) cut or direct activities to specific genomic loci, yet each has off-target activities that are often unpredictable. We developed a pair of simple in vitro assays to systematically measure the DNA-binding specificity (Spec-seq), catalytic activity specificity (S...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Zhang, Liyang, Rube, H Tomas, Vakulskas, Christopher A, Behlke, Mark A, Bussemaker, Harmen J, Pufall, Miles A
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2020
Sujets:
Nucleic Acid Enzymes
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7229833/
https://ncbi.nlm.nih.gov/pubmed/32315032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa231
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