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Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand
Rings of single-stranded RNA are promising for many practical applications, but the methods to prepare them in preparative scale have never been established. Previously, RNA circularization was achieved by T4 RNA ligase 2 (Rnl2, a dsRNA ligase) using splints, but the yield was low due to concurrent...
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| Udgivet i: | Nucleic Acids Res |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7229815/ https://ncbi.nlm.nih.gov/pubmed/32232357 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa181 |
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