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Terminal hairpin in oligonucleotide dominantly prioritizes intramolecular cyclization by T4 ligase over intermolecular polymerization: an exclusive methodology for producing ssDNA rings
When oligonucleotide bearing a hairpin near either its 3′- or 5′-end was treated with T4 DNA ligase, the intramolecular cyclization dominantly proceeded and its monomeric cyclic ring was obtained in extremely high selectivity. The selectivity was hardly dependent on the concentration of the oligonuc...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2018
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6294566/ https://ncbi.nlm.nih.gov/pubmed/30169701 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky769 |
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