DNA 5-Methylcytosine-Specific Amplification and Sequencing

DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC ret...

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Bibliografiske detaljer
Udgivet i:J Am Chem Soc
Main Authors: Liu, Chang, Cui, Xiaolong, Zhao, Boxuan Simen, Narkhede, Pradnya, Gao, Yawei, Liu, Jun, Dou, Xiaoyang, Dai, Qing, Zhang, Li-Sheng, He, Chuan
Format: Artigo
Sprog:Inglês
Udgivet: 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7216556/
https://ncbi.nlm.nih.gov/pubmed/32077696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.9b12707
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