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Quantification of the affinities of CRISPR–Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences
The CRISPR/Cas9 nucleases have been widely applied for genome editing in various organisms. Cas9 nucleases complexed with a guide RNA (Cas9–gRNA) find their targets by scanning and interrogating the genomic DNA for sequences complementary to the gRNA. Recognition of the DNA target sequence requires...
में बचाया:
| में प्रकाशित: | J Biol Chem |
|---|---|
| मुख्य लेखकों: | , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
American Society for Biochemistry and Molecular Biology
2020
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7212624/ https://ncbi.nlm.nih.gov/pubmed/32241913 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.012239 |
| टैग : |
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