Enzymatic Cleavage of 3’-Esterified Nucleotides Enables a Long, Continuous DNA Synthesis

The reversible dye-terminator (RDT)-based DNA sequencing-by-synthesis (SBS) chemistry has driven the advancement of the next-generation sequencing technologies for the past two decades. The RDT-based SBS chemistry relies on the DNA polymerase reaction to incorporate the RDT nucleotide (NT) for extra...

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Foilsithe in:Sci Rep
Main Authors: LinWu, Shiuan-Woei, Tsai, Ting-Yueh, Tu, Yu-Hsuan, Chi, Hung-Wen, Tsao, Yu-Ping, Chen, Ya-Chen, Wang, Hsiang-Ming, Chang, Wei-Hsin, Chiou, Chung-Fan, Lee, Johnsee, Chen, Cheng-Yao
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group UK 2020
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Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7200780/
https://ncbi.nlm.nih.gov/pubmed/32372056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-64541-z
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