Thermococcus sp. 9°N DNA polymerase exhibits 3′-esterase activity that can be harnessed for DNA sequencing

It was reported in 1995 that T7 and Taq DNA polymerases possess 3′-esterase activity, but without follow-up studies. Here we report that the 3′-esterase activity is intrinsic to the Thermococcus sp. 9°N DNA polymerase, and that it can be developed into a continuous method for DNA sequencing with dNT...

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Publicado no:Commun Biol
Main Authors: LinWu, Shiuan-Woei, Tu, Yu-Hsuan, Tsai, Ting-Yueh, Maestre-Reyna, Manuel, Liu, Mu-Sen, Wu, Wen-Jin, Huang, Jyun-Yuan, Chi, Hung-Wen, Chang, Wei-Hsin, Chiou, Chung-Fan, Wang, Andrew H.-J., Lee, Johnsee, Tsai, Ming-Daw
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6586783/
https://ncbi.nlm.nih.gov/pubmed/31240262
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-019-0458-7
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