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Site specific enzymatic cleavage of RNA.
The hybridization of a DNA oligonucleotide a specific tetramer or longer) will direct a cleavage by RNase H (EC 3.1.4.34) to a specific site in RNA. The resulting fragments can then be labeled at their 5' or 3' ends, purified, and sequenced directly. This procedure is demonstrated with two...
Gorde:
| Argitaratua izan da: | Nucleic Acids Res |
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| Egile nagusia: | |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Oxford University Press
1979
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328004/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/386279/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/7.1.179 |
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