A Tandem Guide RNA-Based Strategy for Efficient CRISPR Gene Editing of Cell Populations with Low Heterogeneity of Edited Alleles

CRISPR/Cas9–based gene knockouts (KOs) enable precise perturbation of target gene function in human cells, which is ideally assessed in an unbiased fashion by molecular omics readouts. Typically, this requires the lengthy process of isolating KO subclones. We show here that KO subclones are phenotyp...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:CRISPR J
Autores principales: Joberty, Gérard, Fälth-Savitski, Maria, Paulmann, Marcel, Bösche, Markus, Doce, Carola, Cheng, Aaron T, Drewes, Gerard, Grandi, Paola
Formato: Artigo
Lenguaje:Inglês
Publicado: Mary Ann Liebert, Inc., publishers 2020
Materias:
Research Articles
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7194318/
https://ncbi.nlm.nih.gov/pubmed/32315231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2019.0064
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares