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A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
A single chain fragment variable (scFv) antibody gene was isolated from hybridoma cell line secreting monoclonal antibody (MAb) 20E9 that recognises bluetongue virus (BTV) VP7. DNA fragments encoding variable regions of heavy and light chains were amplified by RT-PCR and library of scFv was construc...
Gespeichert in:
| Veröffentlicht in: | J Virol Methods |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Elsevier Science B.V.
2001
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7172176/ https://ncbi.nlm.nih.gov/pubmed/11164502 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(00)00266-4 |
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